{"id":15,"date":"2017-07-20T12:12:40","date_gmt":"2017-07-20T17:12:40","guid":{"rendered":"https:\/\/pages.stolaf.edu\/colee\/?page_id=15"},"modified":"2024-08-01T10:56:45","modified_gmt":"2024-08-01T15:56:45","slug":"the-biology-of-tetrahymena","status":"publish","type":"page","link":"https:\/\/pages.stolaf.edu\/colee\/the-biology-of-tetrahymena\/","title":{"rendered":"The Biology of Tetrahymena"},"content":{"rendered":"\n

Tetrahymena thermophila is a unicellular, ciliated freshwater protozoan.  It is well suited for undergraduate research in that cells can be grown overnight to densities of 100,000 cells per ml or more.  These cells exhibit a wide repertoire of behaviors that serve as model systems for investigative analysis.<\/p>\n\n\n\n

The “Searchable Ciliate<\/a>” is a website that can serve as a resource for accessing information about Tetrahymena<\/em>‘s rich biological legacy.<\/p>\n\n\n\n

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Mating pair of Tetrahymena labeled to show cytoskeleton {antibody to acetylated alpha tubulin (green)} <\/em>
and micronuclear chromosomes<\/em>
{antibody to phosphorylated micronuclear Histone H1 (red)}.<\/em><\/strong><\/figcaption><\/figure>\n<\/div>\n\n\n

Our laboratory has embarked upon a molecular\/ genetic analysis of both Pattern Formation during the cell’s asexual reproduction (by binary fission) and the mating biology of Tetrahymena <\/em>during Conjugation. Tetrahymena<\/em> can be induced to mate in large synchronous cultures. Nuclear and chromosomal activities can be monitored with DAPI staining and fluorescence microscopy, or in real-time with Nomarski interference microscopy.   Membrane and cytoskeletal dynamics can be followed using indirect immuno-fluorescence or in living cells by driving expression of a variety of GFP-tagged gene products.<\/p>\n\n\n\n

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Gallery<\/a><\/div>\n<\/div>\n\n\n\n
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Projects<\/a><\/div>\n<\/div>\n\n\n\n
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Publications<\/a><\/div>\n<\/div>\n\n\n\n
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Former Students<\/a><\/div>\n<\/div>\n\n\n\n
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Intellectual Ancestry<\/a><\/div>\n<\/div>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

Tetrahymena thermophila is a unicellular, ciliated freshwater protozoan.  It is well suited for undergraduate research in that cells can be grown overnight to densities of 100,000 cells per ml or more.  These cells exhibit a wide repertoire of behaviors that serve as model systems for investigative analysis. The “Searchable Ciliate” is a website that can … <\/p>\n

Continue reading “The Biology of Tetrahymena”<\/span><\/a><\/p>\n","protected":false},"author":1176,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-15","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/pages.stolaf.edu\/colee\/wp-json\/wp\/v2\/pages\/15","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/pages.stolaf.edu\/colee\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/pages.stolaf.edu\/colee\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/pages.stolaf.edu\/colee\/wp-json\/wp\/v2\/users\/1176"}],"replies":[{"embeddable":true,"href":"https:\/\/pages.stolaf.edu\/colee\/wp-json\/wp\/v2\/comments?post=15"}],"version-history":[{"count":36,"href":"https:\/\/pages.stolaf.edu\/colee\/wp-json\/wp\/v2\/pages\/15\/revisions"}],"predecessor-version":[{"id":2898,"href":"https:\/\/pages.stolaf.edu\/colee\/wp-json\/wp\/v2\/pages\/15\/revisions\/2898"}],"wp:attachment":[{"href":"https:\/\/pages.stolaf.edu\/colee\/wp-json\/wp\/v2\/media?parent=15"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}